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Fig. 6. Redistribution of ERGIC53 and COPI upon expression of INPP5B at 15°C. (A) HeLa cells transiently expressing GFP-INPP5B (green) were incubated at 37°C or 15°C for 3 hours, as indicated, before fixation in methanol and labelling with antibodies against β-COP or ERGIC53 (red). Insets show a magnified view of the boxed area. (B) HeLa cells expressing GFP-INPP5B (green) were incubated at 15°C for 3 hours before fixation in methanol and labelling with antibodies against Sec23 or p115 (red). (C) HeLa cells transiently expressing GFP-tagged wild-type INPP5B or the indicated point or truncation mutants (green) were incubated at 15°C for 3 hours, fixed in methanol and labelled for ERGIC53 (red). (D) HeLa cells transiently expressing GFP-INPP5B (green) were incubated at 15°C for 3 hours and either fixed immediately or shifted to 37°C for 10 minutes before fixation and labelling for ERGIC53 (red). Bars, 10 µm.
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