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Figure 3


Fig. 3. Many metaphases in topo-II-depleted cells have one or more elongated chromosome arms stretched towards the spindle pole. (A) A control (expressing topo II{alpha} and IIβ) HTETOP metaphase cell; (B,C) HTETOP topo-II{alpha}-depleted metaphase cells (48 hours exposure to dox); (D,E) topo II{alpha}-depleted (72 hours exposure to dox) and topo IIβ-depleted (72 hours exposure to siRNA) cells arrested in metaphase with MG132 (DNA/DAPI, blue; tubulin, {alpha} or {gamma} as indicated, red; CENP-C, green). Bar, 10 µm.





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