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Figure 4


Fig. 4. Single-molecule force measurements on living KG1a cells. (A) Examples of force-distance curves obtained in the low-adhesion regime (~50x10–18 J, see text). Final ruptures (arrows) were used for further analyses. The retraction speed was set to 2.5 µm/second. (B) Statistical analysis of the rupture forces. The mean rupture forces (± s.e.m.) determined from these data were 58±3 pN and 73±3 pN for the ALCAM-ALCAM and ALCAM-CD6 bond, respectively (P<0.001, n=85; see also Fig. 5). (C) Statistical analysis of the loading rates. The mean loading rates were found to be similar in magnitude (i.e. 2632±112 pN/second and 2707±115 pN/second for the homo- and heterotypic bonds, respectively) (n>100; not significant). A single Gaussian function (solid lines) could be fitted to the force- and loading-rate distributions, which, in all cases, accounted for >85% of the events.





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