spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Hip1r is a phosphorylated protein that associates with membrane and Triton-X100-insoluble fractions. (A) Subcellular cell fractionation of wild-type cells. Most of the cellular pool of Hip1r associates with large membrane structures (LSPs), whereas the rest is soluble (HSS). (B) Triton-X fractionation of Hip1r. Cells were resuspended into buffer containing 0.5% Triton-X100 and fractionated, resulting in a portion of Hip1r sedimenting with the insoluble fraction (P). Extraction of the detergent-insoluble pellet with high-salt buffer shifts Hip1r to the soluble fraction (S). (C) Hip1r is phosphorylated in vivo. Cells were lysed and incubated with calf intestinal phosphatase (CIP) for 25 minutes, resulting in the disappearance of the upper band of the Hip1r doublet. The control lane includes CIP plus okadaic acid, a phosphatase inhibitor. WCL, whole-cell lysate; LSP, low-speed pellet; LSS, low-speed supernatant; HSP, high-speed pellet; HSS, high-speed supernatant; S, supernatant; P, pellet; CIP, calf intestinal phosphatase-treated sample; control, CIP plus okadaic acid.





Right arrow Return to article