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Figure 5


Fig. 5. Hip1r-null spore coats lack organized cellulose. Wild-type and Hip1r-null spores were collected, fixed and stained (see Materials and Methods). (A,B) Wild-type (WT) and Hip1r-null (Hip1r-) spores were collected and washed without detergent. Wild-type spores are elliptical, whereas null spores are round (C,D), and washing wild-type spores and Hip1r-null spores with 0.1% NP40 before fixation results in the collapse of the mutant spores. Bars, 5 µm. (E,F) TEM of spores shows a middle layer of protein arranged in fibrils or crosslinked in wild-type (arrows) spores that is absent for the spore coat of mutant spores. Bar, 500 nm.





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