Supplemental Figure S1
-
Fig. S1. MK-TRAP has no toxicity to NIH3T3 and L cells. (A) L cells were seeded into 96-well dishes and then replaced with 1% FCS/DMEM containing MK-TRAP or control medium after 16 hours of incubation. The cells were counted with a cell counting kit every day for 4 days. The data are shown as the means ± s.e.m. (n=8). (B) The cell growth of NIH3T3 cells was measured every day for 4 days. The data are shown as the means ± s.e.m. (n=8). (C-N) NIH3T3 were cultured in conditioned medium containing MK-TRAP or control medium. After 24 hours or 48 hours of incubation, the apoptosis of cells was measured by the TUNEL method. Counterstaining with DAPI was used to visualize the nuclei. TUNEL, green; DAPI, blue. Serum-free medium (SFM) was used as a negative control, and CDDP (30 μM) was used as a positive control. (C,D) SFM at 24 hours. (E,F) CDDP-containing SFM at 24 hours. (G-J) Control conditioned medium. (K-N) MK-TRAP conditioned medium. C,G,K,E,I,M show DAPI staining, whereas D,H,L,F,J,N show TUNEL staining. (O) Bar graph of the results from C-N. Tunel+/DAPI+ represents the ratio of apoptotic cells to total cells. The data are given as the means ± s.e.m. (n=8).
