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Figure 1


Fig. 1. Ligand blot of MK. (A) Schematic representation of various sLRP fragments. (B) Ligand blot analysis of MK with various fragments. MK was electrophoresed on SDS gels and transferred to a nitrocellulose membrane. Strips of the membranes were then incubated with conditioned medium harvested from sLRP cDNA-transfected COS7 cells. The bound sLRPs were detected using anti-HA antibody. Upper panel, ligand blot result; bottom panel, expression levels of sLRPs. (C) Bar graph of the integrated intensity of each band in (B). The intensities of ligand blot bands were measured and then calculated as the relative intensity (measured intensity/CTN intensity ratio). Experiments were independently performed twice. The results showed the same tendency, and results from one experiment are presented here.





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