spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. MK-TRAP acts by blocking MK-LRP1 interaction. (A) CHO-derived cells were exposed to 125I-MK and treated with the chemical crosslinker BS3. The 125I-MK-crosslinked band appeared in CHO-mLRP4 but not CHO-LRP– cells. MK-TRAP- or sLRP3-containing medium was added to CHO-mLRP4 cells to assess their effects on crosslinking. CHO-LRP cells are deficient in LRP1 expression; CHO-mLRP4 is a stable transfectant expressing LRP1-binding domain IV in the background of CHO-LRP– cells. The arrowhead indicates the unprocessed form of the protein; the arrow indicates the mature form of the protein. (B) GST-RAP, an inhibitor of LRP1, and GST control were administered to M1 and control C1 cells, and the total numbers of colonies in each group were counted and compared by Student's t-test. n=3; error bars, ± s.e.m.; **, P<0.01.





Right arrow Return to article