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Figure 2


Fig. 2. RT-PCR analysis of the mRNAs encoding pannexins and the P2X7 receptor from whole ovary and granulosa cells. (A) Representative results of the analysis for Panx1, Panx2 and Panx3 transcripts. (B) Semiquantitative measurement of Panx1 transcripts in granulosa cells from wild-type (WT) fetuses (E18.5, WT pre-GC), wild-type 3- to 4-week-old mice (WT GC) and Cx43-deficient ovaries 3-4 weeks after grafting into adult kidney capsules (KO GC). The bar graph (bottom) shows the average relative amplicon band intensity (Panx1:Actb amplicon ratio), which was determined by densitometry (n=4; P>0.05, one way ANOVA). (C) Similarly, semiquantitative RT-PCR was used to compare P2rx7 transcript levels in wild-type and Cx43-deficient granulosa cells (n=4; P>0.05, unpaired t-test).





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