JCS016972 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Localisation of POLG and TFAM in cardiac and neuronal precursors derived from spontaneous differentiation of D3 mESCs. (A) Co-labelling of the mesodermal (brachyury; green) and neuroectodermal markers (vimentin, nestin and β-tubulin III; green) with the mtDNA replication factors (POLG and TFAM; red). (B) Proportion of vimentin⁺, nestin+, β-tubulin III⁺ and brachyury⁺ cells in undifferentiated D3 mESCs and days 1-7 of spontaneously differentiated D3 mESCs. (C) Immunocytochemistry for β-tubulin III and POLG on day 20 of differentiation demonstrated the ability of these mESCs to differentiate into neurons. Bars represent mean ± s.e.m.; significant differences between days are indicated (*P<0.05, **P<0.01, ***P<0.001). Bar, 100 μm.

• Supplemental Figure S2 -

  Fig. S2. Expression and localisation of neuroectodermal (vimentin, nestin and β-tubulin III) markers and the mtDNA replication factors (POLG and TFAM) during RA-induced differentiation of D3 mESCs. (A) Co-labelling of vimentin, nestin and β-tubulin III (green) with POLG and TFAM (red). (B) Percentage of vimentin⁺, nestin⁺, and β-tubulin III⁺ cells in undifferentiated and RA-induced D3 mESCs (days 1-7). (C) Dual-immunocytochemistry for β-tubulin III and TFAM on day 20 of differentiation showed extensive staining for TFAM in neurons differentiated from RA-induced D3 mESCs. Bars represent mean ± s.e.m.; significant differences between days are indicated (*P<0.05, ***P<0.001). Bar, 100 μm.