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Figure 1


Fig. 1. Loss of pluripotency during spontaneous differentiation of R1 mESCs. Expression of the pluripotency-associated genes Dppa5, Pramel7 and Ndp52l1 was quantified on undifferentiated (Und) and spontaneously differentiated R1 mESCs (days 1-8) using RT-PCR relative quantification. Expression of individual genes was compared with the expression in undifferentiated R1 mESCs and samples were normalised to Gapdh. Bars represent mean ± s.e.m.; significant differences between days are indicated (*P<0.05, ***P<0.001).





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