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Figure 4


Fig. 4. Detection and localisation of mtDNA, and of the MT-CO1 and POLG proteins, during spontaneous differentiation of D3 mESCs. (A) Immunofluorescence analysis of BrdU (red) and MitoTracker (green) on undifferentiated (Und) and spontaneously differentiated D3 mESCs (days 1-7). Percentages represent the proportion of cells with an expanded cytoplasm at each time point. (B) Immunofluorescence analysis of MT-CO1 (green) and POLG (red) on undifferentiated (Und) and differentiated D3 mESCs (days 1-7). Blue, DNA. Bar, 10 µm.





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