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Figure 1


Fig. 1. P190 immunostaining colocalizes with the CPC in human cells. (A) Immunofluorescence staining of mitotic HeLa cells shows P190 immunoreactivity (green) on kinetochores from prophase to metaphase, in the midzone during anaphase to telophase and in the midbody at the completion of mitosis. With the exception of residual midbody staining, no P190 immunoreactivity is observed during interphase. (B) Nocodazole treatment results in a large number of cells in prophase that progress through mitosis after nocodazole has been removed for 40 or 80 minutes. (C) Dual immunofluorescence shows that P190 (green) colocalizes with Aurora-B (red) throughout mitosis. (D,E) P190 immunoreactivity (green) colocalizes with INCENP (red) in HMECs (D) but not in mouse RAW macrophages (E). DNA is labeled with DAPI (blue). Bars, 10 µm.





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