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Figure 3


Fig. 3. Validation of proteomic results. (A) SMAD2 and phospho-SMAD2 are specifically immunoprecipitated from HeLa mitotic cell lysates using the P190 antibody. (B) SMAD2 phosphorylation on the P190 motif is increased in nocodazole (N)-arrested versus thymidine (T)-arrested HeLa cells. (C) FZR1 is immunoprecipitated by P190, but CDC27, which contains a related motif, is not. (D) RIC8A (red) faintly colocalizes with P190 (green) during prophase and metaphase, and the amount of colocalization increases at later stages of cell division. (E) DLG1 (green) localizes to the cleavage furrow. DNA is labeled with DAPI (blue). Bars, 10 µm.





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