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Figure 1


Fig. 1. In vivo analysis of Myo52 movement. (a) Live timelapse analysis of the Myo52-cGFP strain revealed slow short movements (yellow and green arrows) and fast long directed movements (red, white and blue arrows). Myo52 foci are often seen to change poleward direction of travel (white arrow). (b,c) Cells expressing either Myo52-nGFP or GFP-Atb2 (arrows) were mixed and Myo52 movements were monitored in the (b) absence or (c) presence of 25 µg/ml carbendazim. Microtubule depolymerisation did not affect Myo52 movement. (d-f) Myo52 movements and the actin-patch movement were monitored simultaneously in cells expressing either Myo52-nGFP or Crn1-GFP (arrows) when they were incubated in the presence of (d) DMSO, (e) 2 µM or (f) 20 µM latrunculin A. (g-j) Kymographs of 100x100 msecond timelapse frames of Myo52-cGFP cells treated with (g) DMSO, (h) carbendazim, (i) 2 µM or (j) 20 µM latrunculin A demonstrate that rapid long-distance Myo52 movements are not affected by microtubule depolymerisation but abolished in the absence of actin cables. Bars, 10 µm.





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