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Figure 3


Fig. 3. Myo52 function and movement are not dependent upon its neck region. (a) Myo52 protein with boxed IQ-motif sequences. Residues deleted from GFP-Myo52{Delta}IQ are underlined. (b) Yeast two-hybrid assay showing interaction of the Myo52 tail with itself or the fission yeast calmodulin Cam1. (c) Anti-GFP western blot of myo52{Delta}-strain extracts expressing Myo52-nGFP, GFP-Myo52{Delta}IQ or GFP-Myo52{Delta}CC confirm the expected reduced size of the Myo52 mutant in the latter strain. (d,e) Micrographs of (d) Myo52{Delta}CC and (e) Myo52{Delta}IQ cells show that only Myo52{Delta}IQ localised normally and was able to complement the morphology defect associated with the myo52{Delta} allele. (f) Kymographs of Myo52{Delta}IQ cells demonstrate that the truncation mutant can make rapid directed and bidirectional movements throughout the cell. (g) Velocity distributions of Myo52 and Myo52{Delta}IQ. Bars, 10 µm.





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