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Figure 2


Fig. 2. Protein import into mitochondria lacking Tom70. (A) Import of Su9-DHFR. 35S-labelled Su9-DHFR (comprising the first 69 residues of Neurospora crassa ATP synthase subunit 9 fused to mouse dihydrofolate reductase) was imported into yeast mitochondria isolated from a strain lacking the import receptor Tom70 ({Delta}tom70) or from the corresponding wild-type strain (WT). For calculation of the import efficiency, the total amount of the radiolabelled preprotein in the samples was set to 100%. (B) Import of wild-type DIC into mitochondria from the same preparation as in A. (C) Import of DIC{Delta}CS1/2 into mitochondria from the same preparation as in A. Bars represent standard deviation.





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