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Figure 3


Fig. 3. Expression of Cx43 at the oocyte surface in connexin-replacement follicles. (A) Detection of Cx43 in isolated oocytes by western blot. Oocyte lysates from transgenic lines 2b and 4 along with lysates from Cx37-deficient (Cx37 KO) and wild-type (WT) oocytes were separated electrophoretically, blotted, and the blot probed sequentially with Cx43 antibody (top) and GAPDH antibody (bottom row). (B) Localization of Cx43 in sections of follicles. Whereas no Cx43 was detected at the oocyte surface in wild-type or Cx37-deficient follicles, small foci of Cx43 immunoreactivity typical of gap junction plaques (arrowheads) were detected at the oocyte surface in Cx37-deficient follicles carrying the Cx43-expressing transgene. Bar, 20 µm.





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