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Figure 1


Fig. 1. Human ESCs are resistant to Fas-mediated apoptosis (in contrast to Jurkat cells) independently of their state of differentiation. (A) Human ESCs were incubated with increasing concentrations (0, 50, 100 and 200 ng/ml) of an activating anti-Fas antibody for 24 (white bars) and 48 (black bars) hours. Treatment of ESCs with 200 µg/ml mitomycin C (Mito) was used as a positive control. **P<0.01 for both 24 and 48 hours mitomycin (Mito) treated versus the respective untreated control. (B) Jurkat cells were also incubated with increasing concentrations of an activating anti-Fas antibody for 24 hours. **P<0.01 for treated cells versus untreated control. (C) Undifferentiated (white bars) and decidualized (black bars) human ESCs were incubated with increasing concentrations (0, 50, 100 and 200 ng/ml) of an activating anti-Fas antibody for 24 hours. The rate of apoptosis was measured by flow-cytometric detection of hypodiploid nuclei. Bars show the mean ± s.e.m.





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