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Figure 4


Fig. 4. IFN-{gamma} and TNF-{alpha} cause an upregulation of Fas expression in human ESCs. Human ESCs were treated with 50 ng/ml IFN-{gamma}, 25 ng/ml TNF-{alpha} and 1 U/ml hCG alone and in various combinations for 24 hours (mRNA) and 48 hours (protein) and were subsequently analyzed for the expression of Fas. (A) Fas mRNA level was determined by semiquantitative real-time RT-PCR (*P<0.05: 6 versus 0). (B) The amount of membrane-bound Fas was determined by flow cytometry (**P<0.01: each 6 and 7 versus 0-5). The expression level as measured by MCFI is shown. The signal derived from the untreated control was set to 100. Bars show the mean ± s.e.m.





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