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Figure 5


Fig. 5. Caspase 8 is expressed in human ESCs but is not regulated by IFN-{gamma}, TNF-{alpha} or hCG. (A) ESCs were stained intracellularly to determine total caspase 8 levels and were analyzed by flow cytometry (filled histogram, caspase 8 staining; white histogram, isotype control). (B) ESCs were treated with 50 ng/ml IFN-{gamma}, 25 ng/ml TNF-{alpha} and 1 U/ml hCG alone and in various combinations for 24 hours and were subsequently analyzed for the expression of caspase 8 by flow cytometry. The expression level as measured by MCFI is shown. The signal derived from the untreated control was set to 100. Bars show the mean ± s.e.m.





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