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Figure 6


Fig. 6. PC induces phosphorylation of p53 at Ser15. (A) Time course of PC-induced p53-P in HCT116 cells. HCT116 cells were treated with 200 µM of PCs containing polyunsaturated fatty acids (18:2n6), and p53-P levels were analyzed at varying time points using western blotting. (B) Dose-dependent induction of p53-P by L-{alpha}-PC. U2OS (GW33) cells were treated with varying concentrations of L-{alpha}-PC for 3 hours and p53-P levels were analyzed by western blotting. (C) Effect of PCs containing saturated and monounsaturated fatty acids (8 hours) on p53-P in HCT116 cells. (D) PC induction of p53 phosphorylation requires ATR. ATR-kd-inducible U2OS (GK41) cells were induced for expression of ATR-kd with or without doxycycline and then treated with PC alone or a combination of both PC and BEL (7.5 µM) for 8 hours. TUNEL analysis in PC (18:2n6)-treated HCT116 cells. HCT116 cells were treated with 50 J/m2 UV light, and PCs (100 or 200 µM) for 4 and 8 hours and stained with TUNEL (green) and DAPI (blue) followed by the confocal fluorescence microscopy analysis at x63 magnification. In merged images of TUNEL and DAPI staining, the nucleus appear light blue. Only the merged images of TUNEL and DAPI staining were shown in the PC-treated cells.





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