spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Phospho-defective WAVE2 expression does not affect ruffling in response to PDGF. (A) NIH 3T3 cells stably expressing EGFP, EGFP-WAVE2-wild-type (W2 WT) or EGFP-WAVE2-AAA (W2 AAA) were plated on fibronectin-coated glass coverslips overnight in 0.5% serum. PDGF was added where indicated for 25 minutes before cells were fixed, permeabilised and stained with phalloidin (F-actin). Bar, 30 µm. (B) Experiments were performed as for A, then six random microscopic fields were imaged and the number of cells with one or more ruffles were counted. Dark bars (+) represent cells with ruffles, and light-grey bars (–) represent those without ruffles. Statistical analysis with Fisher's exact test revealed no significant differences between cell lines in either starved or stimulated conditions.





Right arrow Return to article