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Figure 4


Fig. 4. WAVE2 phosphorylation following scratch-wounding. (A) Native NIH 3T3 cells (left panels) or NIH 3T3 cells expressing EGFP-WAVE2-wild-type (right panels) were grown to confluence and scratched. Cultures were left for the indicated times before lysis and immunoprecipitation of endogenous WAVE2 or EGFP-WAVE2 with the indicated antibodies. Immunoprecipitates were blotted as shown. Lysates were taken and blotted for phospho-Erk and phospho-Jnk. (B) In addition to the immunoprecipitates shown in A, NIH 3T3 cells expressing EGFP-WAVE2-AAA were also wounded. Immunoprecipitates were resolved next to a sample of lysate (w) and immunoblotted as indicated.





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