spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Phosphorylation of WAVE2 is required to maintain the directional persistence of cell migration. (A) NIH 3T3 cells stably expressing EGFP, EGFP-WAVE2-wild-type (WT) or EGFP-WAVE2-AAA (AAA) were treated with control siRNA (C) and grown to confluence in a chamber slide. Monolayers were scratch-wounded and cell migration was filmed using time-lapse microscopy. Frames were taken from a representative movie (see supplementary material Movies 1-3 for originals) showing migration at the indicated time points. (B) Cells were tracked every 30 minutes from 8-16 hours post-wounding and their paths overlayed. Paths are oriented such that the start point is normalised to the origin and the wound face runs parallel to the x-axis. All tracks from several fields in this representative experiment are shown. (C,D) Experiment performed as for A and B except that cells were treated with WAVE2 siRNA 48 hours before wounding (see supplementary material Movies 4-6 for originals). (E) Cells from the experiment described in panels A-D were plated in parallel and lysates immunoblotted as indicated.





Right arrow Return to article