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Figure 7


Fig. 7. Phosphorylation of WAVE2 regulates polarisation of the Golgi apparatus. (A) Sample images taken 3 hours post wounding of cells expressing the indicated transgene (EGFP-WT refers to wild-type WAVE2 and EGFP-AAA refers to the triple alanine mutant of WAVE2). EGFP + KD indicates EGFP control cells treated with WAVE2 siRNA. The Golgi apparatus was visualised with an antibody against GM130, and F-actin with phalloidin. Bar, 20 µm. (B) The polarisation of the Golgi apparatus was quantified in cells overexpressing either EGFP (GFP), EFGP-WAVE2-WT (GFP-WT): P values derived by Fisher's exact test are indicated below the percentage figures indicating the percentage of cells with a normally polarised Golgi apparatus. (C) Visualisation of disruption of the Golgi apparatus following treatment with Uo126 and SP600125, each at 10 µM (Uo126+SP). (D) Quantitation of the effects of 10 µM Uo126 (Uo) and SP600125 (SP) on polarisation of the Golgi apparatus. Cell number refers to wound-edge cells with a polarised Golgi apparatus. *** indicates a P value <0.0001 for inhibitor-treated cells compared with DMSO-treated controls. P, polarised Golgi; NP, nonpolarised Golgi.





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