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Fig. 1. Rotenone and TTFA induce cell death in HEK 293 and U87 cells over a 72-hour time course. Cell death was quantified as stated in the Materials and Methods section. HEK 293 (A) and U87 (B) cells were treated with 50.0 µM rotenone (R; complex-I inhibitor) or 0.5 mM TTFA (T; complex-II inhibitor) over a 72-hour time course. Error bars represent s.e. from three independent experiments.