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Figure 7


Fig. 7. Blockage of autophagy response failed to affect ROS generation induced by rotenone or TTFA. (A) HEK 293 cells were treated with rotenone (R, 50 µM) or TTFA (T, 0.5 mM) in the presence or absence of 3-MA (2.0 mM), and ROS generation was determined. (B) Expression of beclin 1 and ATG5 was silenced by siRNAs, and cells were treated with rotenone or TTFA as described in Fig. 1. ROS generation was determined as described above. * Represents a lack of significant difference from control conditions (P>0.05).





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