Supplemental Figure S1
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Fig. S1. (A) C2C12 cultivated for 5 days after serum withdrawal. Compared with control (left) no significant difference was detected in cells overexpressing Lbx1 (right). Bars, 25 Μm. (B) Percentage of MyoD-positive cells after differentiation. MyoD-expressing cells were identified immunohistochemically. The ratios of MyoD-positive C2C12 cells were estimated during differentiation. D3 and D5 indicate days after serum withdrawal. White bars, mock-transfected cells; gray bars, Lbx1-transfected cells; n, number of cells. Control: D3, n=9847; D5, n=10763; Lbx1-transfected: D3, n=6180; D5, n=6005.