spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Lbx1 expression in activated satellite cells. (A-C) Satellite cells freshly isolated from EDL muscles were cultured in satellite cell growth medium for 4 days and then immunostained for Lbx1, MyoD, Pax7 and myogenin, and also counter-stained with Hoechst dye 33342 to identify nuclei. (A) Sphere-shaped cells expressing Pax7, a marker for activated satellite cells, also show high levels of Lbx1 (arrowheads). (B) Sphere-shaped cells expressing MyoD, a marker for activated satellite cells, show high levels of Lbx1 (arrowheads). (C) Sphere-shaped cells that express Lbx1 do not express myogenin (arrowheads). Bars, 50 µm. (D,E) Satellite cells freshly isolated from (D) trunk or (E) diaphragm muscles were cultured in satellite cell growth medium, and then immunostained for Lbx1, Pax7 and MyoD. Note that MyoD- and Pax7-positive satellite cells express Lbx1. Arrowheads indicate Lbx1+/MyoD+ satellite cells (upper panels) or Lbx1+/Pax7+ satellite cells (lower panels). Bars, 10 µm.





Right arrow Return to article