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Fig. 7. Effect of siRNA targeting Lbx1 in primary satellite cells. (A) C2C12 cells transiently expressing Lbx1 were transfected either with siRNA targeting a specific region within Lbx1 or control siRNA. pCX-EGFP was co-transfected with each siRNA. After 48 hours, the cells were immunostained with anti-Lbx1 antibody. Note that Lbx1 expression becomes undetectable in cells transfected with Lbx1 siRNA (arrowheads). Bars 10 µm. (B) Primary satellite cells were co-transfected with Lbx1 siRNA and pCX-nlsEGFP or control siRNA and pCX-nlsEGFP (control). After 24 hours, cells were immunostained using anti-Pax7 antibody. Pax7 could not be detected in cells transfected with Lbx1 siRNA, but was present in those transfected with control siRNA (arrowheads). Bars, 10 µm. (C) Effect of Pax7 knockdown in the satellite cells. Pax7-negative satellite cells were counted in cells used for B. Control (white bars), n=490; Lbx1-siRNA transfected (gray bars), n=444. *P<0.05, statistically significant difference calculated using Student's t-test.
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