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Figure 2


Fig. 2. Lack of apparent colocalisation of Rip11 with markers of early and recycling endosomes, lysosomes and Golgi elements. 3T3-L1 adipocytes were electroporated with plasmids encoding GFP-Rip11 and transferrin receptor (row A), or GFP-Rip11 alone (rows B-F). In row A, the cells were incubated 24 hours later for 30 minutes with transferrin-Alexa Fluor 633 and then fixed and imaged for the localisation of recycling transferrin receptors (red) or GFP-Rip11 (green). In rows B-F, the cells were fixed 24 hours after transfection, permeabilised and stained with antibodies against EEA1 (row B), the mannose 6-phosphate receptor (CI-MPR; row C), LAMP-1 (row D), TGN38 (row E) and giantin (row F). Each row comprises a single cell illustrating the distribution of the relevant marker (red) or GFP-Rip11 (green), with a merged image shown in the right-hand column.





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