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Figure 9


Fig. 9. A Rip11[I630E] mutant retains the ability to inhibit the insulin-stimulated appearance of GLUT4 at the cell surface. 3T3-L1 adipocytes were electroporated with plasmids encoding HA-GLUT4-GFP in the presence of either mRFP vector, mRFP-Rip11 or mRFP-Rip11[I630E], as indicated. 24 hours later, the cells were treated in the absence or presence of 100 nM insulin for 30 minutes and were then fixed and stained with an antibody against HA to detect surface-localised GLUT4. The intensity of HA antibody staining was divided by the intensity of total cellular GFP fluorescence, giving an estimate of the surface appearance of the HA-tagged GLUT4. The data shown are representative of two independent experiments.





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