JCS004580 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. Transfection efficiency determined by flow cytometry. HeLa cells were transfected with non-immune IgG isotype control, anti-RPA32S4^PS8^P or Nbs1 SP343 antibody-Chariot complexes. Transfection efficiencies averaged approximately 80%. The transfection efficiencies shown are 85% and 89% for anti-RPA32S4^PS8^P and -Nbs1S^P343, respectively.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Cell cycle analysis following protein transfection with Chariot reagent. HeLa cells were mock-transfected or transfected with non-immune IgG-Chariot complexes, fixed at the indicated times and cell cycle analysis was performed.

• Supplemental Figure S3 (Adobe PDF) -

  Fig. S3. HU toxicity in NBS-ILB1 cells and transgenes following HU treatment. Cells were treated with 5 mmol HU for 3 and 8 hours; for the 8-hour time point, HU-containing medium was replaced by regular medium after 3 hours and cells were incubated for additional 5 hours. Cell survival was analyzed using the Live/Dead assay.

• Supplemental Figure S4 (Adobe PDF) -

  Fig. S4. H2AX and cell cycle analysis of cells either mock-treated or treated with HU for 3 hours. Bivariate analysis was performed with a BD-FACSArray demonstrating H2AX phosphorylation predominantly in S-phase of HU-treated cells.