spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Effects of FAK mutants on Elk-Gal-reporter and–0.4{alpha}2(I) collagen promoter activity induced by TGFbeta1. (A) Mesangial cells were transfected with the Elk-Gal reporter system as described for Fig. 4C, along with an expression vector for either wild-type FAK (WT) or point mutants (Y397F or Y925F) of FAK, and TGFbeta1 induction of ERK activity was evaluated as Elk-mediated luciferase activity. (B)–0.4{alpha}2(I) collagen promoter activity was stimulated by TGFbeta1 in the presence of either a wild-type or point-mutant (Y397F or Y925F) FAK. A representative result (mean ± s.e.m.) performed in triplicate from one of four separate experiments is shown. Values were corrected for beta-galactosidase expression. Fold induction by TGFbeta1 in each condition is shown in the graphs. White bars, vehicle; black bars, TGFbeta1. *P<0.05, compared to each control by a post-hoc analysis.





Right arrow Return to article