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Figure 2


Fig. 2. Interaction of Tyr and Tyrp1 in melanocytes. Melan-a melanocytes were incubated in ice-cold PBS containing 0, 0.5 or 1 mM DSP (as indicated) for 15 minutes on ice, followed by quenching with 2 mM glycine/PBS and exhaustive washing with PBS. After solubilization, equal amounts of each cell extract were immunoprecipitated and melanogenic proteins were analyzed by SDS-PAGE and immunoblotting. Immunoprecipitated and coprecipitated proteins were separated by SDS-PAGE under reducing conditions, which cleaves the crosslinks, and proteins migrate at their original molecular sizes. Subscripts of `GP' or `R' refer to antisera generated in guinea pigs or rabbits, respectively; NS, normal serum.





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