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Figure 2


Fig. 2. Morphology of embryos obtained from SM22-Cre-TβRIIfl/fl mice. (A-D) SM22-Cre-TβRIIfl/fl littermate embryos collected at E9.5. (A) Wild type (WT); (B) wild type stained for β-galactosidase (blue); (C) SM22-Cre-TβRIIfl/fl embryo; (D) SM22-Cre-TβRIIfl/fl embryo stained for β-galactosidase (blue). (A,C) Arrowheads indicate the well-developed vasculature in the yolk sac. (E-H) SM22-Cre-TβRIIfl/fl littermate embryos collected at E12.5. (E) Wild type; (F) SM22-Cre-TβRIIfl/fl embryo and yolk sac showing vasculature defects when compared with wild type (arrowhead in E); (G) SM22-Cre-TβRIIfl/fl embryo exhibiting defects in the heart; (H) SM22-Cre-TβRIIfl/fl embryo approximately 1-day retarded. (H) Arrowhead indicates underdeveloped heart. e, eye; ht, heart; s, somites. Scale bars, 0.5 mm.





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