spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Depletion of myosin VI or LMTK2 inhibits delivery of TfR to the Rab11-positive perinuclear recycling compartment. (A) HeLa cells stably expressing GFP-Rab11 were transfected with siRNA targeting myosin VI or LMTK2 and processed for immunofluorescence with antibodies against (a,d,g) TfR and (b,e,h) GFP, and labelled with DAPI to visualise nuclei. Merged images are shown in c, f and i. Bar, 10 µm. (B) HeLa cells transfected with siRNA targeting myosin VI or LMTK2 were pulsed with Tf–Alexa-Fluor-647 at 37°C for 30 minutes, washed and incubated at 37°C in the presence of excess of unlabelled transferrin. The amount of Tf–Alexa-Fluor-647 per cell was determined by FACS analysis. Data are presented as the mean ± s.e. from three independent experiments, each performed in duplicate.





Right arrow Return to article