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Figure 4


Fig. 4. Cell-released nucleotides contribute to basal cell proliferation. Steady-state concentration of extracellular ATP and its metabolites measured in the medium of FRT and HeLa cells in control conditions (A; n=10) and after a 1-hour incubation with 2 U/ml apyrase (B; n=5). ATP and ADP induce cell proliferation and both become undetectable after apyrase treatment. (C) Endogenous nucleotides acting through P2Y1Rs participate in the regulation of basal cell proliferation (n=5). Basal [3H]thymidine incorporation in FRT and HeLa cells measured after 16 hours of incubation decreased by about 15-25% in the presence of 1 µM MRS2179 (P2Y1R antagonist) or 2 U/ml apyrase. All results were normalized against the control. Values are the means ± s.e.m. *P<0.05, **P<0.01, Dunnett's test compared with control. Carrier culture medium only was added for the control.





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