JCS015875 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Alternation of the fluorescence of MiDASes. (A) The fluorescence of MiDASes alternated in each daughter half, with a period of ∼24.2±5.3 seconds (n=10). (B) Time-course of fluorescence of left-right pairs of sequential images from the white boxes in panel A. (C) Graph showing alternation of the area of MiDASes in left and right halves. The white and black arrowheads show the peaks of the area in each MiDAS. This phenomenon occurred in ∼20% of the cells examined (44 cells). Bar, 10 µm.

• Movie 1 -

  Movie 1. Dynamics of the MiDASes. Large actin-containing structures were observed underneath the nuclei and centrosomes during mitosis. They split into two and moved into each daughter cell.