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Figure 2


Fig. 2. Distribution of MiDASes, nuclei and centrosomes in multinucleated HS1 cells. Shown is a series of vertical optical sections of a representative multinucleated HS1 cell during cell division. To visualize F-actin and nuclei (A-G), HS1 cells expressing GFP-histone1 (green), which represents the position of nuclei, were fixed and stained with tetramethyl rhodamine-phalloidin (red). (H-N) The distribution of F-actin (red) and GFP–{alpha}-tubulin (green). The optical sections are 1.5 µm in thickness and separated from each other by 0.6 µm (A-F,H-M). Panels G and N show 3D-reconstructed images from panels A-F and H-M, respectively. The positions of MiDASes (arrows) correspond to those of nuclei and centrosomes. Bars, 10 µm.





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