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Figure 3


Fig. 3. Centrosomes and astral microtubules determine the position of MiDASes. (A) MiDASes and centrosomes were observed simultaneously in live HS1 cells expressing mCherry-actin and GFP–{alpha}-tubulin. (B) Sequential images in the box of panel A. The centroid of the MiDAS (closed circle) relocated, following the movement of the centrosome (open circles). (C) Trajectories of a centrosome (green line) and a MiDAS (red line) observed for 99.9 seconds. Arrowheads indicate the original positions and black arrows indicate the final positions of the centrosome and the MiDAS, respectively. (D) TIRF microscopy of a typical dividing multinucleated cell possessing four MiDASes, showing the distal ends of microtubules (green) in the MiDAS regions (arrows). These images were captured by an exposure of 100 mseconds. (E) Sum of traces of microtubules (green) and outlines of MiDASes (red) acquired for 15 seconds, indicating that the distal ends of microtubules reaching to the ventral cell cortex are limited mainly to the MiDAS regions. Bars, 2 µm.





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