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Figure 4


Fig. 4. Astral microtubules are required for the formation and maintenance of MiDASes. HS1 cells expressing GFP–{alpha}-tubulin (A) or GFP-actin (B) were exposed to thiabendazole, an inhibitor of microtubules, under confocal microscopy. Shortly after the addition of thiabendazole, astral microtubules shortened (A), and MiDASes (arrowheads in B) gradually regressed in size and finally disappeared (0-65 seconds in panel B). When thiabendazole was removed by washing with buffer 65 seconds after addition of thiabendazole, MiDASes gradually reappeared underneath the centrosomes (105-160 seconds). The arrows indicate the restored MiDASes (160 seconds). Bars, 5 µm.





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