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Figure 7


Fig. 7. Actin rapidly turns over in MiDASes. The fluorescence of a part of a MiDAS region (within black squares) was bleached in HS1 cells expressing GFP-actin (A). The fluorescence of the bleached region recovered shortly after photobleaching (B). The half-time of recovery was 2.15±0.89 seconds (mean ± s.d., n=17). (C,D) A high-magnification view of the fluorescence recovery experiments. (D) Images inside the square in panel C. After photobleaching, fluorescence appeared as individual small fluorescent dots (arrowheads) and finally formed an aggregate. Bar, 2 µm.





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