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Figure 8


Fig. 8. MiDASes comprise dots of actin. HS1 cells expressing GFP-actin were observed by TIRF microscopy. In the early stage of mitosis, a nascent MiDAS (arrowhead) was formed by aggregation of many small actin dots (A). These small actin dots did not change their positions; they appeared and then disappeared at the same position (panel A, arrows). By contrast, during the final stage of cytokinesis, these actin dots in MiDASes gradually disappeared, and the size of the MiDAS decreased gradually (B). Bar, 2 µm.





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