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Figure 5


Fig. 5. ADF colocalizes with F-actin in the cell body during cell polarization. During cell polarization, cells were extracted with detergent (see Materials and Methods) to remove soluble ADF then fixed and co-stained with Alexa-Fluor-594-conjugated phalloidin (A,B, green) and anti-chick ADF antibody (C,D, red), and the two respective staining patterns were merged (E,F). (A,C,E,E') A re-spread cell with a non-oriented meshwork of F-actin. (B,D,F-F'') A cell with localized cell-edge retraction and oriented actin-filament bundles. Boxed areas in the merged images (E,F) are enlarged in E'-F'', respectively, and show colocalization (orange/yellow) of ADF and the actin meshwork (E') and in a proportion (F'), but not all (F''), of the oriented actin-filament bundles. Bar, 6 µm in A for A-F; 1.3 µm in E',F',F''.





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