JCS012179 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Osmotic shock induced cytofission. The medium of svkA knockout cells was changed to low-salt phosphate buffer and within 60 minutes the huge cells partially separated. The connecting cytoplasmic bridges remained even after the cells formed loose aggregates (800 minutes).

• Movie 2 -

  Movie S2. Chemotaxis of svkA-knockout cells. The cells are moving towards a capillary filled with cAMP (bottom right). This movement is hindered in several cases by a leash that connects the cells and also sticks to the glass surface. Note also the big cell (top right) that moves slowly towards the cAMP source.

• Movie 3 -

  Movie S3. Midwife-assisted cell division. svkA-knockout cells expressing GFP-histone are dividing, and a midwife cell turns and assists in the final cleavage.

• Movie 4 -

  Movie S4. GFP-cortexillin I in svkA-knockout cells. Cortexillin is distributed asymmetrically in the cleavage furrow of a svkA-knockout cell. This leads to problems in severing the connection between the daughter cells.