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Figure 3


Fig. 3. Expression and the activity of Cdk5 are increased following differentiation of OLs. (A) After induction of differentiation, FBD-102b cells were lysed and immunoblotted with an anti-Cdk5 antibody (upper panel). RT-PCR analysis for Cdk5 mRNA was performed in FBD-102b cells after induction of differentiation (lower panel). The internal control β-actin is also shown. (B) After induction of differentiation, endogenous Cdk5 proteins were immunoprecipitated from the lysates of FBD-102b cells with anti-Cdk5 antibody, incubated with buffer containing histone H1 as the substrate, and immunoblotted with an antibody specific for a phosphorylated consensus-Cdk5 motif. The band intensity of phosphorylated histone H1 was quantified. (C) After induction of differentiation, primary OLs were lysed and immunoblotted with anti-Cdk5 antibody. (D) Activity of endogenous Cdk5 in primary OLs was measured after induction of differentiation using histone H1 as the substrate. The band intensity of phosphorylated histone H1 was quantified.





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