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Fig. 5. Cdk5 phosphorylation of paxillin mediates differentiation of OLs. (A) Amino acid sequences of human, mouse and rat paxillin. The consensus Cdk5 phosphorylation sequences are underlined. (B) FBD-102b cells were transfected with plasmid encoding GFP or the GFP-paxillin mutants, and the morphologies of the cells were observed using fluorescence microscopy. Representative fluorescence images of cells are shown. Arrows indicate cell adhesion. (C) Number of processes of cells transfected with the paxillin mutants. Data were evaluated using Student's t-test (*P<0.01). (D) FBD-102b cells were transfected with plasmid encoding GFP or the GFP-paxillin mutants. At 4 days after induction of differentiation, lysates from transfected cells were immunoblotted with antibodies against GFP and β-actin. The asterisk indicates a non-specific band. (E) Primary OPCs were infected with retrovirus expressing GFP, GFP-wt-paxillin, GFP-paxillin-S83A or GFP-paxillin-S244A. Representative fluorescence images of MBP- and GFP-positive cells are shown 3 days after induction of differentiation. Arrows indicate GFP-expressing cells. (F) Percentages of MBP-positive cells (red) against the total number of infected cells. Data were evaluated using Student's t-test (*P<0.01).
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