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Figure 6


Fig. 6. Cdk5 forms a complex with paxillin. (A) In 293T cells, the immunoprecipitates of FLAG-Cdk5 were immunoblotted with an anti-GFP antibody to detect GFP-tagged paxillin. The total protein levels of Cdk5 and paxillin are also shown. (B) 293T cells were co-transfected with plasmids encoding LD1, LD2, LD3, LD4/5, LIM1, LIM2, LIM3 or LIM4 domains of paxillin and FLAG-Cdk5. Lysates of the cells were immunoprecipitated with anti-FLAG antibody and then immunoblotted with anti-HA antibody. Expression of paxillin domains and Cdk5 is also shown. Boxed bands indicate specific binding. (C) In 293T cells, the immunoprecipitates of GFP-wt-paxillin and GFP-paxillin-S244A were immunoblotted with anti-FAK antibody. The total protein levels of FAK and paxillin are also shown.





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